Strutt, David, Professor

Professor David Strutt

School of Biosciences

Wellcome Senior Research Professor

Wellcome Trust Senior Fellow in Basic Biomedical Science

d.strutt@sheffield.ac.uk
+44 114 222 2372

Firth Court

Full contact details

Professor David Strutt
School of Biosciences
Firth Court
Western Bank
91ֱ��
S10 2TN

Profile

2005: Professor of Developmental Genetics
2003-present: Wellcome Senior Fellow in Basic Biomedical Science, University of 91ֱ��
1998-2003: Lister Institute-Jenner Research Fellow, University of 91ֱ��
1997-1998: Lecturer, University of 91ֱ��
1993-1997: Postdoctoral Fellow, European Molecular Biology Laboratory, Heidelberg
1991-1993: Postdoctoral Fellow, Department of Anatomy, University of Cambridge
1988-1991: PhD, Department of Anatomy, University of Cambridge
1985-1988: BA Natural Sciences, University of Cambridge

Research interests

We are interested in the genetic control of animal development and how cells interact to build complex tissues and organs. We focus on the particular problem of how cells coordinate their polarity within developing tissues, and as a model study the phenomenon of planar polarity in epithelia of the fruitfly Drosophila.

Understanding mechanisms of coordinated cell polarisation during animal development

Lay summary of research

We are interested in the process of morphogenesis, which is the process by which a body grows and develops to form complex tissues and organ systems. This involves the regulation and coordination of three processes: first, the multiplication of cells; second, the differentiation of the right types of cells in the right positions; third, the correct orientation of cells relative to each other. It is this final process in which we are primarily interested.

As the orientation of cells relative to each other is a fundamental conserved process in animal development, we are able to study it using model experimental systems, in particular the fruitfly Drosophila melanogaster. Using this organism we can easily manipulate the function of genes, which has allowed the identification of a large number of genes that are involved in the coordination of cell orientation.

The aims of our research are to understand how these genes work together and how they are organised into hierarchies. By understanding how the genes act, we hope to be able to intervene to correct developmental diseases caused by dysfunction of the genes, and also gain insights into conditions such as cancer metastasis which involve coordinated changes in cell polarity.

Scientific summary of research

Morphogenesis – the formation of multidimensional structures from cells and groups of cells – is one of the fundamental processes underlying the development of complex multicellular organisms. A key property underlying many morphogenetic processes is the ability of cells to coordinate their polarity within a sheet of cells, a phenomenon referred to as "planar polarity" or "planar cell polarity [PCP]". Planar polarity enables the concerted cell movements and rearrangements necessary to shape tissues, and allows the production of arrays of polarised structures such as hairs and cilia.

Work in the Strutt lab seeks to understand molecular mechanisms of planar polarisation. Our approach is to study two conserved mechanisms, the "core" Frizzled-dependent planar polarity pathway and the Fat/Dachsous pathway. Our goal is to dissect the feedback and cell-cell communication mechanisms that mediate coordinated cellular symmetry breaking, and ask how this can be aligned with the axes of tissues.

We exploit the traditional strengths of molecular genetic analysis in Drosophila to precisely manipulate gene and protein function in vivo, taking advantage of the minimal genetic redundancy and the easy accessibility of developing tissues. In particular, this system allows us to perform in vivo cell biology, analysing protein dynamics and behaviour in the context of developing tissues.

To do this, we employ both conventional high resolution in vivo imaging techniques such as confocal microscopy, methodologies such as FRAP (fluorescence recovery after photobleaching) and also super-resolution microscopy. These in vivo studies of protein behaviour are combined with studies of the biochemical interactions of pathway components, targeted genetic screens to identify novel regulatory factors, and computational modelling.

Our long term goal is a fundamental understanding of how coordinated cell polarisation is achieved through the integrated spatiotemporal interactions of pathway components acting from the molecular (nanometer) to cellular (micrometre) to tissue (millimetre) scales.

Examples of planar polarised structures on the adult cuticle of the fruitfly Drosophila. Top: photomicrograph of an adult wing (left) with inset (right) showing the regular array of planar polarised hairs on the surface of the wing. Bottom: Scanning electron micrograph of an adult eye (left) with inset (right) showing a histologically stained microtome section through the eye revealing the regular planar polarised arrangements of the groups of cells within the eye that form each facet.

Publications

Show: Featured publications All publications

Featured publications

Journal articles

Strutt H, Warrington S, Madathil ACK, Langenhan T & Strutt D (2023) . Current Biology, 33(24), 5340-5354.e6.
Brittle A, Warrington SJ, Strutt H, Manning E, Tan SE & Strutt D (2022) . Cell Reports, 40(13).
Tan SE, Tan W, Fisher KH & Strutt D (2021) . Development, 148(18).
Strutt H, Gamage J & Strutt D (2019) . eLife, 2019(8).
Strutt H, Langton PF, Pearson N, McMillan KJ, Strutt D & Cullen PJ (2019) . Current Biology, 29(.), 484-491.e6.
Ressurreição M, Warrington S & Strutt D (2018) . Cell Reports, 25(6), 1415-1424.e6.
Warrington SJ, Strutt H, Fisher KH & Strutt D (2017) . Current Biology, 27(18), 2784-2797.e3.
Strutt H, Gamage J & Strutt D (2016) . Cell Rep, 17(10), 2660-2671.
Strutt H, Warrington SJ & Strutt D (2011) . Dev Cell, 20(4), 511-525.

All publications

Journal articles

Strutt H, Meshram D, Manning E, Madathil ACK & Strutt D (2024) . Cell Rep, 43(10), 114722.
Maib H, Adarska P, Hunton R, Vines JH, Strutt D, Bottanelli F & Murray DH (2024) . Journal of Cell Biology, 223(6).
Seehra RS, Warrington SJ, Allouis BHK, Sheard TMD, Spencer ME, Shakespeare T, Cadby A, Bose D, Strutt D & Jayasinghe I (2023) . Cell Reports Physical Science, 4(12).
Strutt H, Warrington S, Madathil ACK, Langenhan T & Strutt D (2023) . Current Biology, 33(24), 5340-5354.e6.
Moreno MR, Hunton R, Strutt D & Bulgakova NA (2023) . Molecular Biology of the Cell.
Brittle A, Warrington SJ, Strutt H, Manning E, Tan SE & Strutt D (2022) . Cell Reports, 40(13).
Mieszczanek J, Strutt H, Rutherford TJ, Strutt D, Bienz M & Gammons MV (2022) . J Cell Sci.
Tan SE, Tan W, Fisher KH & Strutt D (2021) . Development, 148(18).
Strutt H & Strutt D (2021) . Open Biology, 11(2), 200356-200356.
Strutt H & Strutt D (2020) . PLoS Genetics, 16(8).
Gerondopoulos A, Strutt H, Stevenson NL, Sobajima T, Levine TP, Stephens DJ, Strutt D & Barr FA (2019) . Current Biology, 29(19), 3323-3330.e8.
Chaudhary V, Hingole S, Frei J, Port F, Strutt D & Boutros M (2019) . Development, 146(15).
Fisher KH, Strutt D & Fletcher AG (2019) . iScience, 17, 49-66.
Strutt H, Gamage J & Strutt D (2019) . eLife, 2019(8).
Strutt H, Langton PF, Pearson N, McMillan KJ, Strutt D & Cullen PJ (2019) . Current Biology, 29(.), 484-491.e6.
Fisher KH & Strutt D (2019) . Development, 146(3).
Ressurreição M, Warrington S & Strutt D (2018) . Cell Reports, 25(6), 1415-1424.e6.
Fisher KH, Strutt D & Fletcher AG (2017) . Current Opinion in Systems Biology, 5, 41-49.
Warrington SJ, Strutt H, Fisher KH & Strutt D (2017) . Current Biology, 27(18), 2784-2797.e3.
Strutt H, Gamage J & Strutt D (2016) . Cell Rep, 17(10), 2660-2671.
Strutt D, Schnabel R, Fiedler F & Prömel S (2016) . Handb Exp Pharmacol, 234, 249-274.
Saavedra P, Brittle A, Palacios IM, Strutt D, Casal J & Lawrence PA (2016) . Biol Open, 5(4), 397-408.
Strutt H & Strutt D (2015) . Curr Biol, 25(21), R1032-R1034.
Hale R & Strutt D (2015) . Annu Rev Genet, 49, 529-551.
Hale R, Brittle AL, Fisher KH, Monk NAM & Strutt D (2015) . Elife, 4.
Thomas C & Strutt D (2014) . Dev Biol, 385(1), 107-121.
Wells RE, Barry JD, Warrington SJ, Cuhlmann S, Evans P, Huber W, Strutt D & Zeidler MP (2013) . Development, 140(18), 3858-3868.
Strutt H, Thomas-MacArthur V & Strutt D (2013) . PLoS Genet, 9(7), e1003654.
Johnstone K, Wells RE, Strutt D & Zeidler MP (2013) . PLoS One, 8(5), e65076.
Abley K, De Reuille PB, Strutt D, Bangham A, Prusinkiewicz P, Marée AFM, Grieneisen VA & Coen E (2013) . Development, 140(10), 2061-2074.
Strutt H, Searle E, Thomas-Macarthur V, Brookfield R & Strutt D (2013) . Development, 140(8), 1693-1702.
Warrington SJ, Strutt H & Strutt D (2013) . Journal of Cell Science, 126(5), e1-e1.
Warrington SJ, Strutt H & Strutt D (2013) . Development, 140(5), 1045-1054.
Strutt D, Madder D, Chaudhary V & Artymiuk PJ (2012) . Genetics, 192(4), 1295-1313.
Brittle A, Thomas C & Strutt D (2012) . Curr Biol, 22(10), 907-914.
Thomas C & Strutt D (2012) . Dev Dyn, 241(1), 27-39.
Goodrich LV & Strutt D (2011) . Development, 138(10), 1877-1892.
Strutt H, Warrington SJ & Strutt D (2011) . Dev Cell, 20(4), 511-525.
Brittle AL, Repiso A, Casal J, Lawrence PA & Strutt D (2010) . Curr Biol, 20(9), 803-810.
Strutt D & Vincent J-P (2010) . Sci Signal, 3(148), pe43.
Strutt D (2009) . Cold Spring Harb Perspect Biol, 1(5), a000489.
Strutt H & Strutt D (2009) . Semin Cell Dev Biol, 20(8), 957-963.
Strutt H & Strutt D (2008) . Curr Biol, 18(20), 1555-1564.
Strutt D (2008) . Curr Biol, 18(19), R898-R902.
Strutt D & Warrington SJ (2008) . Development, 135(18), 3103-3111.
Brunk K, Vernay B, Griffith E, Reynolds NL, Strutt D, Ingham PW & Jackson AP (2007) . J Cell Sci, 120(Pt 20), 3578-3588.
Bastock R & Strutt D (2007) . Development, 134(17), 3055-3064.
Strutt D & Strutt H (2007) . Dev Biol, 302(1), 181-194.
Strutt H, Price MA & Strutt D (2006) . Curr Biol, 16(13), 1329-1336.
Shimada Y, Yonemura S, Ohkura H, Strutt D & Uemura T (2006) . Dev Cell, 10(2), 209-222.
Strutt H & Strutt D (2005) . Bioessays, 27(12), 1218-1227.
Strutt D (2005) . Curr Biol, 15(18), R758-R759.
Strutt D (2005) . Dev Cell, 8(2), 134-136.
Strutt H & Strutt D (2005) . Advances in Developmental Biology, 14, 39-57.
Strutt H, Mundy J, Hofstra K & Strutt D (2004) . Development, 131(4), 881-890.
Strutt D (2003) . Development, 130(19), 4501-4513.
Strutt H & Strutt D (2003) . Curr Biol, 13(16), 1451-1457.
Bastock R, Strutt H & Strutt D (2003) . Development, 130(13), 3007-3014.
Strutt H & Strutt D (2002) . Dev Cell, 3(6), 851-863.
Strutt H & Strutt D (2002) . Curr Biol, 12(11), R384-R385.
Strutt DI (2002) . Semin Cell Dev Biol, 13(3), 225-231.
Strutt D, Johnson R, Cooper K & Bray S (2002) . Curr Biol, 12(10), 813-824.
Strutt D (2001) . Curr Biol, 11(13), R506-R509.
Strutt DI (2001) . Mol Cell, 7(2), 367-375.
Zeidler MP, Perrimon N & Strutt DI (2000) . Dev Biol, 228(2), 181-196.
Fanto M, Weber U, Strutt DI & Mlodzik M (2000) . Curr Biol, 10(16), 979-988.
Zeidler MP, Perrimon N & Strutt DI (1999) . Curr Biol, 9(23), 1363-1372.
Strutt H & Strutt D (1999) . Curr Opin Genet Dev, 9(4), 442-446.
Zeidler MP, Perrimon N & Strutt DI (1999) . Genes Dev, 13(10), 1342-1353.
Boutros M, Paricio N, Strutt DI & Mlodzik M (1998) . Cell, 94(1), 109-118.
Strutt DI & Mlodzik M (1997) Hedgehog is an indirect regulator of morphogenetic furrow progression in the Drosophila eye disc.. Development, 124(17), 3233-3240.
Strutt DI, Weber U & Mlodzik M (1997) . Nature, 387(6630), 292-295.
Strutt DI & Mlodzik M (1996) . Mech Dev, 58(1-2), 39-50.
Strutt DI & Mlodzik M (1995) Ommatidial polarity in the Drosophila eye is determined by the direction of furrow progression and local interactions.. Development, 121(12), 4247-4256.
Strutt DI, Wiersdorff V & Mlodzik M (1995) . Nature, 373(6516), 705-709.
Strutt DI & White RA (1994) . Mech Dev, 46(1), 27-39.
White RA, Brookman JJ, Gould AP, Meadows LA, Shashidhara LS, Strutt DI & Weaver TA (1992) . J Cell Sci Suppl, 16, 53-60.
Gould AP, Brookman JJ, Strutt DI & White RA (1990) . Nature, 348(6299), 308-312.
Harrison C, Shao H, Strutt H & Strutt D () . Biochemical Society Transactions.

Chapters

Warrington SJ, Strutt H & Strutt D (2022) , Methods in Molecular Biology (pp. 1-30). Springer US
Strutt H & Strutt D (2005) , PLANAR CELL POLARIZATION DURING DEVELOPMENT (pp. 39-57).

Conference proceedings papers

Wells R, Strutt D & Zeidler M (2009) . MECHANISMS OF DEVELOPMENT, Vol. 126 (pp S176-S176)
Warrington S & Strutt D (2009) . MECHANISMS OF DEVELOPMENT, Vol. 126 (pp S80-S80)
Searle E, Strutt H & Strutt D (2009) . MECHANISMS OF DEVELOPMENT, Vol. 126 (pp S144-S144)
Brown S, Smythe E, Strutt D, Whitworth A & Zeidler M (2009) . MECHANISMS OF DEVELOPMENT, Vol. 126 (pp S290-S290)
Strutt D, Bastock R & Strutt H (2003) Non-canonical Wnt/frizzled signalling and cell polarity in Drosophila. EUROPEAN JOURNAL OF CELL BIOLOGY, Vol. 82 (pp 84-85)

Preprints

Carayon A & Strutt D (2024) , Cold Spring Harbor Laboratory.
Maib H, Adarska P, Hunton R, Vines J, Strutt D, Bottanelli F & Murray DH (2023) , Cold Spring Harbor Laboratory.
Tan SE, Tan W, Fisher KH & Strutt D (2020) , Cold Spring Harbor Laboratory.
Strutt H & Strutt D () , Cold Spring Harbor Laboratory.
Fisher KH, Strutt D & Fletcher AG () .

Grants

Wellcome Trust Senior Fellowship in Basic Biomedical Science: ‘Planar polarity as a model for understanding self-organisation from molecular to tissue scales’
UKRI/Wellcome Trust – Building Collaboration at the Physics of Life Interface: Dr Alex Fletcher and Prof David Strutt (joint-PIs), Prof Ashley Cadby (co-I), ‘Understanding self-organised tissue patterning across scales’

Teaching activities: Undergraduate:
• BIS218 Advanced Developmental Biology Practical Sessions

Professional activities and memberships

Member of DBT/Wellcome Trust India Alliance Intermediate and Senior Fellowships Selection Committee (2021–)
Member of Wellcome Trust Cell and Developmental Biology Expert Review Group (2017–2020)
Member of Human Frontiers Science Program Grants Committee (2013–2017)
Visiting scientist Kavli Institute for Theoretical Physics (KITP), University of California, Santa Barbara (2013, 2016, 2023)
Editorial boards of Current Biology and Development Member of the British Society for Developmental Biology and the Genetics Society