Professor Andrew Furley
School of Biosciences
Professor of Developmental Neuroscience, Deputy Head of School,
+44 114 222 2354
Full contact details
School of Biosciences
D18a
Firth Court
Western Bank
91直播
S10 2TN
- Profile
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- 2021-Present: Deputy Head of School, School of Biosciences, University of 91直播
- 2018-2021: Professor of Developmental Neuroscience, School of Biosciences, University of 91直播. Head of Research Training Faculty of Science
- 2004-2018: Senior Lecturer, Biomedical Science, University of 91直播. Head of Research Training, Faculty of Science
- 1997-2004: Lecturer (Centre for Developmental & Biomedical Genetics), Biomedical Science, University of 91直播
- 1992-1997: MRC Research Scientist, Division of Developmental Neurobiology, NIMR, Mill Hill, London
- 1990-1992: Howard Hughes Medical Institute Fellow, Center for Neurobiology & Behavior, Columbia University, New York (Advisor: T. Jessell)
- 1987-1990: Jane Coffin Childs Post-Doctoral Fellow, Dept of Biochemistry, Columbia University, New York (Advisors:F. Alt/T. Jessell)
- 1987: PhD Leukaemia Biology, Imperial Cancer Research/UCL, London (Supervisor: M. Greaves)
- 1982: B.Sc. (Hons) Molecular Biology, University of Edinburgh
- Research interests
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Our research is focussed on the role L1CAM-like cell adhesion molecules (L1-CNTNs) in neural development and disease (; ). L1-CNTNs affect neural function at all stages, including the earliest proliferation and differentiation of progenitor and stem cells (; ; ; ; ), the guidance of axons (; ; ), through to firing of action potentials () and functioning of synapses (). As a result, these molecules are widely implicated in neurological disease and cancers.
Our aim is to understand the cellular mechanisms through which L1-CNTNs affect this wide variety of processes. Our current work is focussed particularly on the role of NrCAM in regulating the Sonic Hedgehog (SHH) pathway in medulloblastoma (; Xenaki et al., 2011) and in neural stem cells (), with a specific emphasis on its role in controlling the trafficking of SHH pathway components into and out of primary cilia ().
Most recently, we have begun to use neural organoids derived from human induced pluripotent cells (iPSCs) to develop in vitro systems with which to study both L1-CNTN function and to generate hypothalamic progenitors and neurons (with Barbaric and Placzek in BMS).
The cell biology of signal modulation in neural development and cancer
In biology context is everything. We aim to understand how cellular context alters the responses of cells to intercellular signals, focussing on the role of the L1-contactin 鈥榓dhesion鈥 molecules (L1-CNTNs) in neural development. We have shown L1-CNTNs to modulate signals as diverse as semaphorins and hedgehogs (HHs) by altering the intracellular trafficking of their receptors during signalling. Our work on L1-CNTN modulation of Sema signalling during the wiring of mouse spinal sensory circuits () has converged on our studies showing F3/contactin repression of Sonic HH-stimulated proliferation of cerebellar neuron progenitors () to focus on a potential role for NrCAM in the trafficking of SHH signalling pathway components in the primary cilium. We are collaborating with Cadby (Physics) to develop STORM super resolution microscopy to follow this in real time (). Collaborations with Clifford (Newcastle) reveal NrCAM to be upregulated in SHH group medulloblastomas and loss of NrCAM in vivo affects the penetrance of neoplasia in the Ptch1 mouse medulloblastoma model ().
Roles for NrCAM in hypothalamic stem cell behaviour and for TAG1 in autism and obesity (3) are also being investigated with Placzek (BMS), Markx (NY) and Buchner (Ohio; ) respectively, while with Whyte, Walmsley (Edinburgh) and Renshaw (IICD/Bateson) we have investigated semaphorins in neutrophil function ().
I also have a particular interest in the use of animals in research, which relates to my masters Bioethics teaching (with , Maastricht), and was the 91直播 lead in an FP7 project () aimed at evaluating the impact of Directive 2010/63/EU on animal welfare and research competitiveness in member states ().
- Publications
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Show: Featured publications All publications
Featured publications
Journal articles
- . Development, 148(11).
- . Cell Reports, 34(13).
- . PLOS Biology, 18(3).
- . Journal of Clinical Investigation.
- . BMJ Open Science, 3(1).
- . Molecular and Cellular Neuroscience, 81, 1-3.
- . Molecular and Cellular Neuroscience, 81, 49-63.
- . Cereb Cortex, 25(1), 167-179.
- . PLoS ONE, 8, e57960-e57960.
- . J Neurosci, 32(30), 10370-10382.
- . Mamm Genome, 23(7-8), 431-442.
- . Development, 138(3), 519-529.
- . Development, 135(14), 2361-2371.
- . Nat Cell Biol, 10(3), 283-294.
- . Frontiers in Neuroscience, 16.
Conference proceedings papers
All publications
Journal articles
- . Development, 148(11).
- . Cell Reports, 34(13).
- . PLOS Biology, 18(3).
- . Journal of Clinical Investigation.
- . BMJ Open Science, 3(1).
- . Optics Communications, 404, 18-22.
- . Molecular and Cellular Neuroscience, 81, 1-3.
- . Molecular and Cellular Neuroscience, 81, 49-63.
- . Cereb Cortex, 25(1), 167-179.
- . PLoS ONE, 8, e57960-e57960.
- . J Neurosci, 32(30), 10370-10382.
- . Mamm Genome, 23(7-8), 431-442.
- . Developmental Biology, 365(1), 133-151.
- . Development, 138(3), 519-529.
- . Development, 135(14), 2361-2371.
- A TAG1-APP signalling pathway through Fe65 negatively modulates neurogenesis (vol 10, pg 283, 2008). NAT CELL BIOL, 10(4), 497-497.
- . Nat Cell Biol, 10(3), 283-294.
- . Neuroscience, 123(1), 155-166.
- . J Cell Biol, 162(6), 1149-1160.
- . Development, 130(1), 29-43.
- . Mamm Genome, 13(5), 245-252.
- . Genome Biology, 3(3), interactions2001.1-interactions2001.1.
- The trappist's approach to pathfinding: elucidating brain wiring using secretory-trap mutagenesis. Genome biology, 3(3), interactions2001.1-interactions2001.1.
- Overlapping functions of the cell adhesion molecules Nr-CAM and L1 in cerebellar granule cell development (vol 154, pg 1259, 2001). JOURNAL OF CELL BIOLOGY, 155(2), 311-311.
- . Eur J Neurosci, 14(8), 1209-1218.
- . J Cell Biol, 154(6), 1259-1273.
- . Genome Biol, 2(9), REVIEWS1026.
- . Curr Biol, 10(24), 1607-1610.
- . Mol Cell Neurosci, 16(4), 499-514.
- . Mech Dev, 80(2), 197-201.
- A functional interaction between the neuronal adhesion molecules TAG-1 and F3 modulates neurite outgrowth and fasciculation of cerebellar granule cells.. J Neurosci, 18(17), 6853-6870.
- . Curr Biol, 8(1), 26-33.
- . Cell, 90(2), 257-269.
- . Current Biology, 6(5), 526-529.
- . Neuron, 12(3), 675-690.
- . Genomics, 18(3), 562-567.
- . Trends Neurosci, 16(8), 316-323.
- . Trends in Glycoscience and Glycotechnology, 3(13), 360-369.
- . Cell, 61(1), 157-170.
- Separate elements control DJ and VDJ rearrangement in a transgenic recombination substrate.. EMBO J, 9(1), 117-125.
- . Cold Spring Harb Symp Quant Biol, 54 Pt 1, 191-202.
- . Recent Results Cancer Res, 117, 62-66.
- . Cell, 54(4), 453-460.
- Cell lineage specificity of chromatin configuration around the immunoglobulin heavy chain enhancer.. EMBO J, 7(8), 2393-2399.
- MYC FAMILY GENE-EXPRESSION DURING HUMAN THYMIC ONTOGENY. CLINICAL RESEARCH, 36(3), A573-A573.
- . Ann N Y Acad Sci, 546, 9-24.
- Lineage specificity of rearrangement and expression of genes encoding the T cell receptor-T3 complex and immunoglobulin heavy chain in leukemia.. Leukemia, 1(9), 644-652.
- . Acta Paediatr Jpn, 29(4), 539-541.
- . Proc Natl Acad Sci U S A, 84(14), 4999-5003.
- Distribution and epitope analysis of the cell membrane glycoprotein (HPCA-1) associated with human hemopoietic progenitor cells.. Leukemia, 1(5), 417-426.
- . Br J Haematol, 65(3), 277-287.
- . Pediatrics International, 29(4), 539-541.
- . Immunology Today, 8(4), 115-116.
- . Haematol Blood Transfus, 31, 320-323.
- Inappropriate rearrangement of immunoglobulin and T-cell receptor genes. Trends in Immunology, 8(4), 115-116.
- Rearrangement of immunoglobulin heavy chain genes in human T leukaemic cells shows preferential utilization of the D segment (DQ52) nearest to the J region.. EMBO J, 5(13), 3467-3473.
- . Blood, 68(5), 1101-1107.
- Divergent molecular phenotypes of KG1 and KG1a myeloid cell lines.. Blood, 68(5), 1101-1107.
- Differentiation-linked gene rearrangement and expression in acute lymphoblastic leukaemia.. Clin Haematol, 15(3), 621-639.
- . Cell, 46(1), 75-87.
- . Blood, 67(2), 533-536.
- Clonal rearrangement and expression of the T cell receptor beta gene and involvement of the breakpoint cluster region in blast crisis of CGL.. Blood, 67(2), 533-536.
- . Blood, 67(1), 1-11.
- Lineage promiscuity in hemopoietic differentiation and leukemia.. Blood, 67(1), 1-11.
- . Frontiers in Neuroscience, 16.
Chapters
- In Terman J (Ed.), Semaphorin Signalling New York: Humana Press.
- TAG-1/Axonin-1 In Kreis T & Vale R (Ed.), Guidebook to the extracellular matrix and adhesion proteins A Sambrook and Tooze Publication at Oxford University Press
Conference proceedings papers
- . Thorax, Vol. 73(Suppl_4). London, UK, 5 December 2018 - 7 December 2018.
- "Mind the method!" - A longitudinal assessment of methodological soundness and quality of reporting of als research on the sod1g93a mouse. MEDICINE, Vol. 95(10)
- . Cilia, Vol. 4((Suppl 1)) (pp 35-35), 18 November 2015 - 21 November 2015.
- The mRNA exporter GLE1 is essential for embryonic development. FEBS Journal, Vol. 281(Suppl 1) (pp 644-645)
- . MECHANISMS OF DEVELOPMENT, Vol. 126 (pp S306-S306)
- A tag on to the physiological functions of APP in neurogenesis. JOURNAL OF NEUROCHEMISTRY, Vol. 106 (pp 32-32)
- Target-derived nerve growth factor (NGF) regulating spinal sensory axon outgrowth to the embryonic mouse gut. GASTROENTEROLOGY, Vol. 130(4) (pp A255-A255)
- Pathfinding of spinal commissural axons: The role of immunoglobulin-like cell adhesion molecules. EUROPEAN JOURNAL OF NEUROSCIENCE, Vol. 12 (pp 276-276)
- Misexpression of the mouse F3 axonal glycoprotein under the transient axonal glycoprotein promoter results in a cerebellar phenotype in early development.. EUROPEAN JOURNAL OF NEUROSCIENCE, Vol. 12 (pp 275-275)
- Gait analysis for possible behavioral assessment of transgenic mice. FASEB J, Vol. 12(4) (pp A135-A135)
Preprints
- Research group
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External collaborators
- Prof. Steve Clifford, Newcastle
- Prof. Moira Whyte & Dr Sarah Walmsley, Edinburgh
- Prof. Gianfrano Gennarini, Bari, Italy
- Prof. David Townend, Maastricht, Netherlands
- Dr Sander Markx, Columbia, NY, USA
- Dr David Buchner, Case Western, Ohio, USA
- Dr Avihu Klar, Hebrew University, Israel
- Dr David Beier, Harvard Medical School, Boston, USA
Current Lab Members:
- Miss Bethany James (MRC DiMeN PhD Student; joint with Prof Marysia Placzek and Dr Ivana Barbaric)
- Grants
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- Brain Tumour Support across Yorkshire
- FP7
- HEFCE
- Teaching activities
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Undergraduate and postgraduate taught modules
Undergraduate
- BMS109-153 Neuroscience
- BMS243/249 Developmental Neurobiology
- BMS381 Developmental Neurobiology (Co-ordinator)
- BMS382 Stem Cell Biology
- Level 3 Practical and Dissertation Modules
- BMS404 Ethics, the Law and Public Awareness of Science (Co-ordinator)
Masters (MSc)
- BMS6054 - Ethics, the Law and Public Awareness of Science (Co-ordinator)
- BMS6318 Developmental Neurobiology (Co-ordinator)
- Professional activities and memberships
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- British Society for Developmental Biology (BSDB; ) Committee member (1999-2005); Publications Secretary (2000-2005;
- MRC Advisory Board (MAB) 2002-2005
- Appointed adviser on genetics and biotechnology to EU-wide consortium (PRIVILEGED) determining the ethical and legal interests in privacy and data protection for research involving the use of genetic databases and bio-banks, 2007.
- Chair: BNA Symposium, Edinburgh. April 2015
- Panel Member: Volkswagen Foundation DiSCUSS-Cancer Stem Cells, Hannover. Oct 2014
- 2016 - 2020: External Examiner UG & MSc Neuroscience Programme, King鈥檚 College, London
- 2019: Invited participant & organiser, Bioethics Workshop, University of Zurich
- 2016: Guest Editor, Molecular and Cellular Neuroscience, Special Issue on 鈥淐ell Adhesion Molecules in Development and Disease鈥
- 2015: Chair and co-organiser, International Symposium 鈥淓xploring Axonal Development And Connectivity鈥 at British Neuroscience Association Conference, Edinburgh
- 2014: Invited attendance the Academy of Medical Science symposium on 鈥淩eproducibility and Reliability of Biomedical Science鈥 (April 2014) at the Wellcome Trust
- 2014: Career Session for Early Career Neurobiologists and Neuroscientists, Cold Spring Harbor Symposium on Axon Guidance & Neural Plasticity
- 2013-2017: UK lead for FP7 consortium: 鈥楢n ethical, legal and practical perspective on the impact of a new regulatory framework for the scientific use of animals on research and innovation鈥