Craggs, Timothy D., Dr

Dr Timothy D. Craggs

School of Mathematical and Physical Sciences

Senior Lecturer in Biological Chemistry

t.craggs@sheffield.ac.uk
+44 114 222 9347

Dainton Building

Full contact details

Dr Timothy D. Craggs
School of Mathematical and Physical Sciences
Dainton Building
13 Brook Hill
91ֱ��
S3 7HF

Profile: Dr Tim Craggs obtained his MSci in Chemistry from the University of Cambridge in 2002. After his PhD (Cambridge 2007) and a postdoc in St Andrews, he took up a Lindemann Fellowship at Yale University (2010), followed by senior postdoc positions at Oxford (Kapanidis Lab) and Bristol (Dillingham Lab). In 2016 he was appointed to a Lectureship in Chemical Biology at the University of 91ֱ��.

Research interests

Research Keywords: Single Molecule Spectroscopy

Single-molecule approaches provide unprecedented detail to the understanding of essential biological processes, as was recognized in the awarding of the 2014 Nobel Prize for Chemistry. Their unique advantage stems from the ability to go beyond the ensemble- and time-averaging of common biochemical techniques, enabling the identification and interpretation of asynchronous reactions, transient states, and rare sub-species.

Research in the Craggs Lab involves the development and application of single-molecule fluorescence techniques to addressing crucial questions across physics, chemistry and the life sciences.

Recent work has focussed on the development and application of single-molecule fluorescence resonance energy transfer (smFRET – a molecular ruler for the 30-90 Å scale) to questions of protein folding, and DNA transcription, replication and repair. These methods are capable of observing individual molecules and molecular interactions in real time, and understanding their dynamics.

In addition to this mechanistic work, we have shown we can use smFRET to measure absolute distances with angstrom accuracy, opening the door to FRET driven structural biology.

Publications

Journal articles

Akolgo GA, Partridge BM, D. Craggs T & Amewu RK (2023) . BMC Infectious Diseases, 23.
Agam G, Gebhardt C, Popara M, Mächtel R, Folz J, Ambrose B, Chamachi N, Chung SY, Craggs TD, de Boer M , Grohmann D et al (2023) . Nature Methods, 20, 523-535.
Hill VE, Noy A & Craggs TD (2023) . Biophysical Journal, 122(3), 214a-214a.
Rhind-Tutt AV, Fountain SE, Abdelhamid MA & Craggs TD (2023) . Biophysical Journal, 122(3), 19a-20a.
Jeffet J, Ionescu A, Michaeli Y, Torchinsky D, Perlson E, Craggs TD & Ebenstein Y (2022) . Biophysical Journal, 121(3), 301a-301a.
Abdelhamid MAS, Waller ZAE & Craggs TD (2022) . Biophysical Journal, 121(3), 171a-171a.
Ambrose B, Hill VE, Shaw RA, Johnston-Wood T, Willmott M, Johnston C, Mächtel R, Cordes T, Lerner E, Hill JG , Weiss S et al (2022) . Biophysical Journal, 121(3), 431a-431a.
Evans GW, Craggs T & Kapanidis AN (2022) . Journal of Molecular Biology, 434(2).
Bennison DJ, Nakamoto JA, Craggs TD, Milón P, Rafferty JB & Corrigan RM (2021) . mBio, 12(6).
Jeffet J, Ionescu A, Michaeli Y, Torchinsky D, Perlson E, Craggs TD & Ebenstein Y (2021) . Biophysical Reports, 1(1).
George D, Cadby A & Craggs TD (2021) . Biophysical Journal, 120(3), 184a-184a.
Ambrose B, Baxter JM, Cully J, Willmott M, Steele EM, Bateman BC, Martin-Fernandez ML, Cadby A, Shewring J, Aaldering M & Craggs TD (2020) . Nature Communications, 11(1).
Abdu K, Aiertza MK, Wilkinson OJ, Senthong P, Craggs TD, Povey AC, Margison GP & Williams DM (2020) . Nucleosides, Nucleotides & Nucleic Acids.
Ambrose B, Willmott M, Johnston-Wood T, Shaw RA, Hill JG & Craggs TD (2020) . Biophysical Journal, 118(3), 615a-615a.
Ambrose B, Baxter J, Cully J, Willmott M, Bateman BC, Steele E, Cadby AJ, Shewring J, Aaldering M & Craggs TD (2020) . Biophysical Journal, 118(3), 614a-614a.
Craggs TD, Sustarsic M, Plochowietz A, Mosayebi M, Kaju H, Cuthbert A, Hohlbein J, Domicevica L, Biggin PC, Doye JPK & Kapanidis AN (2019) . Nucleic Acids Research, 47(20), 10788-10800.
Martin E, Williams HEL, Pitoulias M, Stevens D, Winterhalter C, Craggs TD, Murray H, Searle MS & Soultanas P (2019) . Nucleic Acids Research, 47(4), 2101-2112.
Hellenkamp B, Schmid S, Doroshenko O, Opanasyuk O, Kühnemuth R, Adariani SR, Ambrose B, Aznauryan M, Barth A, Birkedal V , Bowen ME et al (2018) . Nature Methods, 15(9), 669-676.
Bennet I, Finger LD, Baxter NJ, Ambrose B, Hounslow A, Thompson MJ, Exell JC, Shahari NNBM, Craggs TD, Waltho JP & Grasby JA (2018) . Nucleic Acids Research, 46(11), 5618-5633.
Hellenkamp B, Schmid S, Doroshenko O, Opanasyuk O, Kühnemuth R, Adariani SR, Ambrose B, Aznauryan M, Barth A, Birkedal V , Bowen ME et al (2018) . Nature Methods, 15(11), 984-984.
Fisher GLM, Pastrana CL, Higman VA, Koh A, Taylor JA, Butterer A, Craggs TD, Sobott F, Murray H, Crump MP , Moreno-Herrero F et al (2017) . eLife, 6.
Craggs TD (2017) . Nature Methods, 14(2), 123-124.
Meli M, Sustarsic M, Craggs TD, Kapanidis AN & Colombo G (2016) . Frontiers in Molecular Biosciences, 3.
Nott TJ, Craggs TD & Baldwin AJ (2016) . Nature Chemistry, 8(6), 569-575.
Algasaier SI, Exell JC, Bennet IA, Thompson MJ, Gotham VJB, Shaw SJ, Craggs TD, Finger LD & Grasby JA (2016) . Journal of Biological Chemistry, 291(15), 8258-8268.
Evans GW, Hohlbein J, Craggs T, Aigrain L & Kapanidis AN (2015) . Nucleic Acids Research, 43(12), 5998-6008.
Nott TJ, Petsalaki E, Farber P, Jervis D, Fussner E, Plochowietz A, Craggs TD, Bazett-Jones DP, Pawson T, Forman-Kay JD & Baldwin AJ (2015) . Molecular Cell, 57(5), 936-947.
Blouin S, Craggs TD, Lafontaine DA & Penedo JC (2015) , 115-141.
(2014) . Chemical Society Reviews, 43(17), 6470-6470.
Craggs TD, Hutton RD, Brenlla A, White MF & Penedo JC (2014) . Nucleic Acids Research, 42(3), 1857-1872.
Craggs TD, Sustarsic M, Hohlbein J, Cuthbert A, Taylor N, Evans G & Kapanidis AN (2014) . Biophysical Journal, 106(2), 274a-274a.
Hohlbein J, Craggs TD & Cordes T (2014) Alternating-laser excitation: single-molecule FRET and beyond (vol 43, pg 1156, 2014). CHEMICAL SOCIETY REVIEWS, 43(17), 6472-6472.
Robb NC, Cordes T, Hwang LC, Gryte K, Duchi D, Craggs TD, Santoso Y, Weiss S, Ebright RH & Kapanidis AN (2013) . Journal of Molecular Biology, 425(5), 875-885.
Hohlbein J, Aigrain L, Craggs TD, Bermek O, Potapova O, Shoolizadeh P, Grindley NDF, Joyce CM & Kapanidis AN (2013) . Nature Communications, 4(1).
Craggs TD & Kapanidis AN (2012) . Nature Methods, 9(12), 1157-1158.
Hutton RD, Craggs TD, White MF & Penedo JC (2010) . Nucleic Acids Research, 38(5), 1664-1675.
Craggs TD (2010) . ChemInform, 41(3).
Craggs TD (2009) . Chemical Society Reviews, 38(10), 2865-2865.
Blouin S, Craggs TD, Lafontaine DA & Penedo JC (2009) , 475-502.
Orte A, Craggs TD, White SS, Jackson SE & Klenerman D (2008) . Journal of the American Chemical Society, 130(25), 7898-7907.
Kuprov I, Craggs TD, Jackson SE & Hore PJ (2007) . Journal of the American Chemical Society, 129(29), 9004-9013.
Huang J-R, Craggs TD, Christodoulou J & Jackson SE (2007) . Journal of Molecular Biology, 370(2), 356-371.
Jackson SE, Craggs TD & Huang J-R (2006) . Expert Review of Proteomics, 3(5), 545-559.
Khan F, Kuprov I, Craggs TD, Hore PJ & Jackson SE (2006) . Journal of the American Chemical Society, 128(33), 10729-10737.
Darcy M, Crocker K, Wang Y, Le JV, Mohammadiroozbahani G, Abdelhamid MAS, Craggs TD, Castro CE, Bundschuh R & Poirier MG () . ACS Nano.
Abdelhamid MAS, Rhind-Tutt AV, Ambrose B & Craggs TD () . Journal of Visualized Experiments(173).
Abdelhamid MAS, Rhind-Tutt AV, Ambrose B & Craggs TD () . Journal of Visualized Experiments(173).
Lerner E, Barth A, Hendrix J, Ambrose B, Birkedal V, Blanchard SC, Börner R, Sung Chung H, Cordes T, Craggs TD , Deniz AA et al () . eLife, 10.
Martin E, Williams HEL, Pitoulias M, Stevens D, Winterhalter C, Craggs TD, Murray H, Searle MS & Soultanas P () .
Craggs TD, Sustarsic M, Plochowietz A, Mosayebi M, Kaju H, Cuthbert A, Hohlbein J, Domicevica L, Biggin PC, Doye JPK & Kapanidis AN () .
Fisher GLM, Pastrana CL, Higman VA, Koh A, Taylor JA, Butterer A, Craggs TD, Sobott F, Murray H, Crump MP , Moreno-Herrero F et al () .
Hohlbein J, Craggs TD & Cordes T () . Chem. Soc. Rev., 43(4), 1156-1171.
Lerner E, Ambrose B, Barth A, Birkedal V, Blanchard SC, Borner R, Cordes T, Craggs TD, Ha T, Haran G , Hugel T et al () The FRET-based structural dynamics challenge -- community contributions to consistent and open science practices.
Ambrose B, Baxter J, Cully J, Willmott M, Steele E, Bateman BC, Martin-Fernandez ML, Cadby A, Shewring J, Aaldering M & Craggs TD () .
Shaw R, Johnston-Wood T, Ambrose B, Craggs T & Hill J () . Journal of Chemical Theory and Computation.

Chapters

Tor Y, Gehring K, Fabris D, Egli M, Korostelev AA, Craggs TD, Pyne A, Gagnon KT, Watts JK, Cheatham III TE & Richards NGJ (2022) , Nucleic Acids in Chemistry and Biology (pp. E1-E50). The Royal Society of Chemistry

Conference proceedings papers

Fisher GLM, Bateman BC, Craggs TD & Dillingham MS (2018) . Biophysical Journal, Vol. 114(3) (pp 209a-209a)
Craggs TD, Sustarsic M, Mosayebi M, Kaju H, Hohlbein J, Biggin PC, Doye JPK & Kapanidis AN (2016) . Biophysical Journal, Vol. 110(3) (pp 514a-515a)
Craggs TD, Sustarsic M, Hohlbein J, Cuthbert A, Taylor N, Evans G & Kapanidis AN (2014) A Novel FRET-Based Structure of DNA Polymerase Complexed with Kinked Gapped-DNA. BIOPHYSICAL JOURNAL, Vol. 106(2) (pp 273A-273A)
Orte A, Craggs TD, White SS, Jackson SE & Klenerman D (2007) YFP unfolding kinetics studied in a single-molecule nano-flow cell. BIOPHYSICAL JOURNAL (pp 660A-660A)

Preprints

Howard JAL, Ambrose B, Abdelhamid MAS, Alevropoulos-Borrill A, Ejaz A, Dresser L, Dienerowitz M, Quinn SD, Squires AH, Noy A , Craggs TD et al (2024) , Cold Spring Harbor Laboratory.
Gelder KL, Carruthers NA, Ball SS, Dunning M, Craggs TD, Twelvetrees AE & Bose DA (2024) , Cold Spring Harbor Laboratory.
Agam G, Gebhardt C, Popara M, Mächtel R, Folz J, Ambrose B, Chamachi N, Chung SY, Craggs TD, de Boer M , Grohmann D et al () , Cold Spring Harbor Laboratory.
George DR, Ambrose B, Cadby AJ & Craggs TD () , Cold Spring Harbor Laboratory.
Bennison DJ, Nakamoto JA, Craggs TD, Milón P, Rafferty JB & Corrigan RM () .
Jeffet J, Michaeli Y, Torchinsky D, Israel-Elgali I, Shomron N, Craggs TD & Ebenstein Y () .

Teaching interests: Biological Chemistry

Teaching activities

Undergraduate and postgraduate taught modules

An Introduction to Biology for Physical Scientists (Level 1)
This unit provides a basic knowledge and understanding of the occurrence, structure and function of important types of biopolymers such as proteins and nucleic acids, their organisation into biomaterials and their function in living systems.
Biopolymers and Biomaterials (Post-graduate Level)
This course explores the occurrence, structure and function of important types of biopolymers such as proteins and nucleic acids, their organisation into biomaterials and their function in living systems.

Support Teaching:

Level 3 Literature Review

Laboratory Teaching:

Level 2 Organic Laboratories
Level 3 Research Project
Level 4 Research Project