Green, Luke, Dr

Dr Luke Green

School of Clinical Dentistry

Research Fellow

L.R.Green@sheffield.ac.uk
+44 114 215 9547

Room LU105, L Floor, The Medical School

Full contact details

Dr Luke Green
School of Clinical Dentistry
Room LU105, L Floor
The Medical School
Beech Hill Road
91ֱ��
S10 2RX

Profile

For enquiries, please contact – ClinMed-Operational@sheffield.ac.uk

2021-Present: The Humane Research Trust Research Fellow; University of 91ֱ��, UK.
2018-2021: Postdoctoral Associate; Prof. Pete Monk, University of 91ֱ��, UK.
2015-2018: Postdoctoral Researcher; Dr. Chris Bayliss, University of Leicester, UK.
2011-2015: Research Associate; Dr. Ingrid Scully Group, Pfizer Inc., USA.
2006-2010: PhD; Prof. Rob Read Group, University of 91ֱ��, UK.

Research interests

Infectious disease is a huge health burden globally and antibiotic resistance is an ever growing problem, currently attributable to ~700,000 deaths per year with projected figures rising to 10 million by the year 2050. For this reason, new therapies to replace antibiotics are crucial. Due to their rapid doubling time, bacteria have the ability to quickly evolve mechanisms to overcome drugs targeted to them, for this reason, I believe the future for anti-infectives is to target infection at the human cell level. Bacteria enter our cells by "hijacking" parts of our own cell machinery (proteins).

Our cells are encased in a membrane consisting of water-repelling fatty acids and proteins. Bacteria have to attach to and breach this membrane in order to get inside our cells. They do this by "hijacking" the proteins within the membrane. Whilst there has been extensive research into how bacteria bind our cells, no universal mechanisms (used by all bacteria) are known. Different bacterial species have been shown to hijack different proteins, which causes problems with therapeutic design. I have discovered that a family of human cell membrane proteins, the tetraspanins, are extremely important in the binding of multiple bacterial species to our cells. Targeting tetraspanins using drugs reduces bacterial binding to multiple types of human cells by greater than 50%. The tetraspanins form part of our cell membranes and act as "organisers", bringing together lots of other proteins to form large protein "islands" in the cell membrane. Bacteria do not directly attach to the tetraspanins but I hypothesise that the tetraspanins organize the proteins required for bacterial binding and it is therefore the loss of this organization which results in reduced bacterial adhesion to our cells when the tetraspanins are blocked.

My lab is interested in investigating the role of these proteins and their interactors in bacterial infection.

Publications

Show: Featured publications All publications

Featured publications

Journal articles

Green LR, Issa R, Albaldi F, Urwin L, Thompson R, Khalid H, Turner CE, Ciani B, Partridge LJ & Monk PN (2023) . mBio, e0148223.
Wanford JJ, Holmes JC, Bayliss CD & Green LR (2020) . Microbial Genomics, 6(5).
Green LR, Al-Rubaiawi AA, Al-Maeni MARM, Harrison OB, Blades M, Oldfield NJ, Turner DPJ, Maiden MCJ & Bayliss CD (2020) . mBio, 11(2).
Green LR, Dave N, Adewoye AB, Lucidarme J, Clark SA, Oldfield NJ, Turner DPJ, Borrow R & Bayliss CD (2019) . The Journal of Infectious Diseases, 220(7), 1109-1117.
Green LR, Lucidarme J, Dave N, Chan H, Clark S, Borrow R & Bayliss CD (2018) . Journal of Clinical Microbiology, 56(9).
Oldfield NJ, Green LR, Parkhill J, Bayliss CD & Turner DPJ (2018) . The Journal of Infectious Diseases, 217(4), 608-616.
Green LR, Monk PN, Partridge LJ, Morris P, Gorringe AR & Read RC (2011) . Infect Immun, 79(6), 2241-2249.

All publications

Journal articles

Green LR, Issa R, Albaldi F, Urwin L, Thompson R, Khalid H, Turner CE, Ciani B, Partridge LJ & Monk PN (2023) . mBio, e0148223.
Jadi PK, Dave A, Issa R, Tabbasum K, Okurowska K, Samarth A, Urwin L, Green LR, Partridge LJ, MacNeil S , Garg P et al (2023) . The Ocular Surface.
Dave N, Albiheyri RS, Wanford JJ, Green LR, Oldfield NJ, Turner DPJ, Martinez-Pomares L & Bayliss CD (2023) . Microbiology, 169(2), micro001305.
Parker MD, Stewart H, Shehata OM, Lindsey BB, Shah DR, Hsu S, Keeley AJ, Partridge DG, Leary S, Cope A , State A et al (2022) . Communications Biology, 5.
Green LR, Cole J, Parga EFD & Shaw JG (2022) . Advances in microbial physiology, 80, 35-83.
Lindsey BB, Villabona-Arenas CJ, Campbell F, Keeley AJ, Parker MD, Shah DR, Parsons H, Zhang P, Kakkar N, Gallis M , Foulkes BH et al (2022) . Nat Commun, 13(1), 1013.
Lindsey BB, Villabona-Arenas CJ, Campbell F, Keeley AJ, Parker MD, Shah DR, Parsons H, Zhang P, Kakkar N, Gallis M , Foulkes BH et al (2022) . Nature Communications, 13(1).
Partridge LJ, Urwin L, Nicklin MJH, James DC, Green LR & Monk PN (2021) . Cells, 10(6).
Parker MD, Lindsey BB, Leary S, Gaudieri S, Chopra A, Wyles M, Angyal A, Green LR, Parsons P, Tucker RM , Brown R et al (2021) . Genome Research.
Remmington A, Haywood S, Edgar J, Green LR, de Silva T & Turner CE (2021) . Microbial Genomics, 7(1).
Urwin L, Okurowska K, Crowther G, Roy S, Garg P, Karunakaran E, MacNeil S, Partridge LJ, Green LR & Monk PN (2020) . Cells, 9(11).
Korber B, Fischer WM, Gnanakaran S, Yoon H, Theiler J, Abfalterer W, Hengartner N, Giorgi EE, Bhattacharya T, Foley B , Hastie KM et al (2020) . Cell, 182(4), 812-827.e19.
The COVID-19 Genomics UK (COG-UK) consortium , Wang D, Angyal A, Brown RL, Carrilero L, de Silva TI, Green LR, Groves DC, Keeley AJ, Lindsey BB , Parker MD et al (2020) . The Lancet Microbe, 1(3), e99-e100.
Wanford JJ, Holmes JC, Bayliss CD & Green LR (2020) . Microbial Genomics, 6(5).
Green LR, Al-Rubaiawi AA, Al-Maeni MARM, Harrison OB, Blades M, Oldfield NJ, Turner DPJ, Maiden MCJ & Bayliss CD (2020) . mBio, 11(2).
Green LR, Dave N, Adewoye AB, Lucidarme J, Clark SA, Oldfield NJ, Turner DPJ, Borrow R & Bayliss CD (2019) . The Journal of Infectious Diseases, 220(7), 1109-1117.
Gray K, Green LR, Chaudhuri RR & Shaw JG (2019) . Microbiology Resource Announcements, 8(30).
Dave N, Green L, Lucidarme J, Oldfield N & Bayliss C (2019) . Access Microbiology, 1(1A).
Aidley J, Wanford JJ, Green LR, Sheppard SK & Bayliss CD (2018) . Microbial Genomics, 4(11).
Green LR, Lucidarme J, Dave N, Chan H, Clark S, Borrow R & Bayliss CD (2018) . Journal of Clinical Microbiology, 56(9).
Oldfield NJ, Green LR, Parkhill J, Bayliss CD & Turner DPJ (2018) . The Journal of Infectious Diseases, 217(4), 608-616.
Oldfield NJ, Cayrou C, AlJannat MAK, Al-Rubaiawi AAA, Green LR, Dada S, Steels OD, Stirrup C, Wanford J, Atwah BAY , Bayliss CD et al (2017) . Emerging Infectious Diseases, 23(6), 1009-1011.
Scully IL, Swanson K, Green L, Jansen KU & Anderson AS (2015) . Current Opinion in Microbiology, 27, 96-102.
Green LR, Monk PN, Partridge LJ, Morris P, Gorringe AR & Read RC (2011) . Infect Immun, 79(6), 2241-2249.
Holmes JC, Green LR, Oldfield NJ, Turner DPJ & Bayliss CD () . Frontiers in Genetics, 11.
Wanford JJ, Green LR, Aidley J & Bayliss CD () . PLOS ONE, 13(5), e0196675-e0196675.
Cayrou C, Akinduko AA, Mirkes EM, Lucidarme J, Clark SA, Green LR, Cooper HJ, Morrissey J, Borrow R & Bayliss CD () . PLOS ONE, 13(5), e0197186-e0197186.
Parker MD, Lindsey BB, Leary S, Gaudieri S, Chopra A, Wyles M, Angyal A, Green LR, Parsons P, Tucker RM , Brown R et al () .

Chapters

Green LR, Haigh RD & Bayliss CD (2019) , Methods in Molecular Biology (pp. 83-92). Springer New York
Green LR, Eiden J, Hao L, Jones T, Perez J, McNeil LK, Jansen KU & Anderson AS (2016) , Vaccine Design (pp. 445-469). Springer New York

Preprints

Green LR, Issa R, Albaldi F, Urwin L, Khalid H, Turner CE, Ciani B, Partridge LJ & Monk PN (2023) , Cold Spring Harbor Laboratory.
Parker MD, Lindsey BB, Shah DR, Hsu S, Keeley AJ, Partridge DG, Leary S, Cope A, State A, Johnson K , Ali N et al () , Cold Spring Harbor Laboratory.
Partridge LJ, Urwin L, Nicklin MJH, James DC, Green LR & Monk PN () .

Grants

The Humane Research Trust Research Fellowship.
THRT PhD Scholarship.